goat serum (gs) 5 (Thermo Fisher)

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Thermo Fisher goat serum (gs) 5
Goat Serum (Gs) 5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+serum+(gs)/Newborn+Calf+Serum%2C+New+Zealand+origin/pmc11322642-334-22-26
Average 90 stars, based on 1 article reviews

goat serum (gs) 5 - by Bioz Stars, 2026-10

90/100 stars

Images

Incubation:

Article Title: Altered Ca2+ responses and antioxidant properties in Friedreich's ataxia-like cerebellar astrocytes.
Article Snippet: .. The fixed cells were incubated with primary antibodies (see below) in 10% goat serum (GS), 0.1% Triton X-100 in PBS overnight at 4°C and, subsequently, with anti-rabbit-IgG or anti-mouse-IgG secondary antibodies (1:1000, Invitrogen), and counterstained with DAPI (D9542, Sigma). .. Images were acquired using a Axio Observer (Zeiss Axio Observer.Z1 with Hamamatsu EM9100) and ArrayScan microscope.

other:

Article Title: AAV2/9-mediated gene transfer into murine lacrimal gland leads to a long-term targeted tear film modification.
Article Snippet: For primary antibodies produced in rabbits and chickens, cryosections were blocked with a mixture of 5% goat serum (GS, Thermo Fisher Scientific, reference 16210064), 5% of fish skin gelatin (FSG, Sigma-Aldrich, reference G7765), and 0.1% Triton X-100 in PBS for 1 h at room temperature.

Article Title: Minimal genetically encoded tags for fluorescent protein labeling in living neurons
Article Snippet: Afterward, cells were incubated for 1 h at RT with a blocking serum containing 3% bovine serum albumin (BSA; Sigma-Aldrich, cat. no. A9647), 10% goat serum (GS; ThermoFisher Scientific, cat. no. 16210072), and 0.2% Triton X-100 diluted in PBS.

Article Title: E-cadherin might be a stage-dependent modulator in aggressiveness in pancreatic cancer cells
Article Snippet: This procedure was followed by blocking with 10% goat serum (GS, 31872, Thermo Fisher Scientific Inc., Waltham, MA, USA) for 1 h and conjugating E- cadherin, vimentin, and Snail antibodies (Human EMT-3 Color Immunocytochemistry Kit, Cat. No. SC026, R&D Systems, Inc., Minneapolis, MN, USA) at room temperature for 3 h at a concentration 1:50 in PBS.

Staining:

Article Title: Characterization of the Antibody Response after Cervical Spinal Cord Injury
Article Snippet: Primary goat anti-rat IgM antibody (1:100, Invitrogen) was incubated overnight at 4°C, followed by fluorescent-tagged secondary antibody (Alexa Fluor 488, 1:200; Life Technologies) and 4′,6-diamidino-2-phenylindole (DAPI; 1:400), also overnight at 4°C. .. Next, sections were blocked with 5% goat serum (GS) + 1% BSA + 0.3% Triton X-100 in PBS for 1 h and stained with fluorescent-conjugated anti-rat IgG antibody (Alexa Fluor 568, 1:150; Life Technologies) for 2 h at room temperature. ..

Microscopy:

Article Title: Intracellular autofluorescence enables the isolation of viable, functional human muscle reserve cells with distinct Pax7 levels and stem cell states
Article Snippet: .. Cells were then permeabilized and blocked in PBS containing 5% goat serum (GS), 0.3% Triton X-100 for 30 min, followed by a PBS wash. EdU detection was performed using the Click-iT ® Plus reaction cocktail for 30 min. After final washes, coverslips were mounted onto glass slides using ProLong TM Glass Antifade Mountant with NucBlue TM (Invitrogen, cat# P36981 ) and images were acquired using a Zeiss Axio Imager Z1 microscope. .. Cells were then permeabilized and blocked in PBS containing 5% goat serum (GS), 0.3% Triton X-100 for 30 min, followed by a PBS wash. EdU detection was performed using the Click-iT Plus reaction cocktail for 30 min. After final washes, coverslips were mounted onto glass slides using ProLong Glass Antifade Mountant with NucBlue (Invitrogen, cat# P36981 ) and images were acquired using a Zeiss Axio Imager Z1 microscope.

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